Highly infectious plasmids carrying poliovirus cDNA are capable of replication in transfected simian cells
- 1 August 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 59 (2) , 490-493
- https://doi.org/10.1128/jvi.59.2.490-493.1986
Abstract
We examined events leading to production of infectious poliovirus upon transfection of simian cells with plasmids carrying poliovirus cDNA and simian virus 40 transcription and replication signals. The nature of the simian virus 40 promoter upstream from the poliovirus cDNA had no influence on its infectivity. A high specific infectivity was correlated with plasmid replication, dependent on expression of T antigen either encoded by the plasmid or present in host cells (COS-1).This publication has 24 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Nuclear localization of poliovirus capsid polypeptide VP1 expressed as a fusion protein with SV40-VP1Gene, 1985
- Evidence for the direct involvement of DNA replication origin in synthesis of late SV40 RNANature, 1982
- Cloned Poliovirus Complementary DNA Is Infectious in Mammalian CellsScience, 1981
- In vivo sequence requirements of the SV40 early promoter regionNature, 1981
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981
- Heterogeneity and 5′-terminal structures of the late RNAs of simian virus 40Journal of Molecular Biology, 1978
- Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescueVirology, 1978
- Complete nucleotide sequence of SV40 DNANature, 1978
- Two sequence-specific endonucleases from Moraxella bovisJournal of Molecular Biology, 1977