The sedimentation behaviour of mixtures of hyaluronic acid and albumin in the ultracentrifuge

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Abstract
Experiments were done on the ultracentrifugal sedimentation of hyaluronic acid and protein (albumin) in human synovial fluids, and in human and ox synovial fluid with added albumin. An empirical correction for the effect of albumin on the sedimenta tion of hyaluronic acid is obtained, and applied to the results obtained for human synovial fluids. The effect of albumin on the sedimentation of hyaluronic acid, when the latter is the slower component, is shown to be consistent with the "McFarlane" (1935 a, b) boundary anomaly. The effect of albumin on the sedimentation of hyaluronic acid and the reverse effect, in regions of the cell where both are present, have been investigated. The former is shown to be consistent in general with current theory. The latter effect requires special explanation, and the hypothesis is put forward that a large fraction of the hyaluronic acid particle is not penetrable by albumin.