Effect of Nutrients Upon Growth of Streptomycin-Sensitive, -Resistant-and -Dependent Strains of Escherichia coli.

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Abstract
NaCl, as well as glucose, pyruvate, and fumarate, favored the growth of a streptomycin-dependent strain of E. coli in nutrient broth. The greatest initial growth of this strain took place in the presence of 5 [mu]g./ml. of streptomycin. In the presence of Na salts of pyruvate and fumarate, initial growth took place in higher concns. of streptomycin, namely 25 [mu]g./ml. The growth of the sensitive parent E. coli strain was inhibited by 5 [mu]g./ml. of strepto-mycin. The dependent strain grew in all concns. of streptomycin from 5-1000 [mu]g./ml. but not in media without streptomycin. Two resistant strains were obtained, one of which grew in all concns. of streptomycin as well as in media without streptomycin, while a 2d resistant strain grew only in a streptomycin concn. of 500 [mu]g /ml. with pyruvate and fumarate as carbon sources. The dependent strain gave rise to streptomycin-sensitive cells when a large inoculum was placed in streptomycin-free nutrient broth.

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