A Comparison of Lead, Bismuth, and Iron as Detectors of Hydrogen Sulphide Produced by Bacteria

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Abstract
In peptone media iron salts are more sensitive than lead or bismuth salts for detecting H2S. The ratios of the concentrations which are required to detect sulphides to that which inhibits the growth of bacteria are for Fe 1:40, for Bi 1:5, and for Pb 1:1. Lead acetate papers are 10-100 times more sensitive than the best iron media. The papers are semi-quantitative and with papers, unlike mineral media, results are not obscured by colored media or pigmented bacteria. For the determination of the ability of bacteria to produce H2S lead acetate papers are advocated.

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