The Identification of Low Molecular Weight Bentonite Flocculating Antibodies in the Serum of the Rabbit, Monkey and Man

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Abstract
Summary: Small molecular weight antibodies, capable of specifically flocculating antigen-coated bentonite, have been identified in antisera prepared against endotoxins in the rabbit, monkey and man and against human serum albumin in the rabbit. These antibodies sediment at less than 2S by sucrose density gradient ultracentrifugation and, by gel filtration on Sephadex G-200, have a molecular size slightly larger than that of lysozyme (MW 14,000 to 18,000). By radioimmunoelectrophoresis and zone electrophoresis in starch, these proteins migrate with the more negatively charged antibodies. The low molecular weight antibodies in sera from three volunteers were consistently removed by co-precipitation with antisera specific for γM-globulin and, also, in one specimen with antisera specific for γG-globulin.