Purification of xylanase from the wood-rotting fungusTrametes hirsuta
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 21 (1) , 28-35
- https://doi.org/10.1007/bf02879003
Abstract
Xylanase (EC 3.2.1.8) was purified from a crude extracellular enzyme of the wood-rotting fungusTrametes hirsuta by a four-step procedure involving precipitation with ammonium sulphate, gel filtration on Bio-Gel P-10, column chromatography on DEAE-Sephadex A-50 and preparative electrophoresis on polyacrylamide gel. The isolated enzyme, electrophoretically homogeneous, was separated from β-xylosidase and other hydrolytic enzymes studied. The analysis of the degradation products of water-soluble (4-0-methylglucurono)-D-xylan from willow by the purified xylanase showed it to be endoxylanase (β-1,4-xylan xylanohydrolase).Keywords
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