Comparison of six methylation methods for analysis of the fatty acid composition of albacore lipid
- 1 October 1992
- journal article
- Published by Oxford University Press (OUP) in International Journal of Food Science & Technology
- Vol. 27 (5) , 597-601
- https://doi.org/10.1111/j.1365-2621.1992.tb01228.x
Abstract
Summary: Six methods widely used to produce methyl esters for the gas chromatographic determination of the fatty acid composition of a marine lipid were compared. Four acid‐catalyzed methods (1% H2SO4: CH3OH; 5% HCl: CH3OH; 7% and 14% BF3: CH3OH) and two base‐catalyzed methods [0.5M NaOCH3: CH3OH; (1:4) tetramethylguanidine: CH3OH] were used.The use of BF3: CH3OH (7% and 14%) gave a lower content of 18:1 n9 than the other methods and produced an artefact (2.7–3.2% of total fatty acid content) eluting between the 20:5 and 24:1 fatty acid methyl esters. No significant differences were obtained between the other four methods.Accordingly the use of BF3: CH3OH for transmethylation of marine lipids is not recommended. Results obtained in the other four methods showed that all are comparable.Keywords
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