Cloning and chromosomal location of the alpha- and beta-globin genes from a marsupial.
- 1 December 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (23) , 8105-8108
- https://doi.org/10.1073/pnas.82.23.8105
Abstract
The cDNA sequences encoding the adult .alpha.- and .beta.-globin polypeptides of a marsupial, the native cat Dasyurus viverrinus, have been cloned and their nucleotide sequences have been determined. Using these cDNA clones we have determined the chromosomal location of the native cat .alpha.- and .beta.-globin genes by in situ hybridization to fixed metaphase chromosomes and by hybridization to DNA extracted from chromosomes purified by a fluorescence-activated cell sorter. Using two independent methods of gene assignment we have shown the .alpha.- and .beta.-globin-like gene sequences to be asyntenic in the native cat, the .alpha.-globin-like sequences being on chromosome 2 and the .beta.-globin-like sequences being on chromosome 4.This publication has 18 references indexed in Scilit:
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