Deficient DNA repair of triple helix‐directed double psoralen damage in human cells
- 30 October 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 374 (2) , 287-291
- https://doi.org/10.1016/0014-5793(95)01133-y
Abstract
Damage induced by a single psoralen‐modified triple helix‐forming oligonucleotide has been reported to be efficiently repaired in human cells. In this study we investigated a set of psoralen coupled oligonucleotides introducing multiple lesions into the target DNA. A simian virus 40 (SV40) shuttle vector was in vitro treated with different triple helix‐forming oligonucleotides and UVA radiation, leading to double psoralen adducts at the supF mutational target gene of the plasmid. After passage in the Raji human cell line the recovered vector was analysed in an indicator bacterial strain. The results show that double psoralen adducts, located at both ends of a long triple helix, cannot be repaired efficiently in human cells.Keywords
This publication has 13 references indexed in Scilit:
- The Action ofEscherichia coliEndonuclease III on Multiply Damaged Sites in DNAJournal of Molecular Biology, 1995
- Triplex-mediated inhibition of HIV DNA integration in vitro.Journal of Biological Chemistry, 1994
- Reversible inhibition of gene expression by a psoralen functionalized triple helix forming oligonucleotide in intact cellsJournal of Biological Chemistry, 1994
- Differential Regulation of Gene Expressionin Vivoby Triple Helix-Forming Oligonucleotides as Detected by a Reporter EnzymeAntisense Research and Development, 1994
- Repair of triple helix directed psoralen adducts in human cellsNucleic Acids Research, 1994
- Oligo-α-deoxyribonucleotides with a modified nucleic base and covalently linked to reactive agentsTetrahedron, 1993
- Effect of sequence, adduct type, and opposing lesions on the binding and repair of ultraviolet photodamage by DNA photolyase and (A)BC excinucleaseJournal of Biological Chemistry, 1993
- DNA triple‐helix formation: An approach to artificial gene repressors?BioEssays, 1992
- Inhibition of Simian Virus 40 DNA replication in CV-1 cells by an oligodeoxynucleotide covalently linked to an intercalating agentNucleic Acids Research, 1989
- Site-Specific Oligonucleotide Binding Represses Transcription of the Human c- myc Gene in VitroScience, 1988