Studies on antitumor polysaccharides, especially D-II, from mycelium of Coriolus versicolor.

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Abstract
A water-soluble polysaccharide, D-II, with marked antitumor activity was isolated from the cultured mycelium of C. versicolor by extraction with hot water, fractional precipitation with ethanol and ion-exchange chromatography. D-II strongly inhibited the growth of [mouse] sarcoma 180 cells transplanted s.c. in mice by i.p., i.v., s.c. or i.m. administration at a dose of 5 mg/kg. The MW was estimated to be 2 .times. 106 by gel-filtration or 6.5 .times. 106 by light scattering analysis. The chemical structure of D-II was investigated by periodate oxidation, methylation analysis, Smith degradation and a combination of controlled Smith degradation and methylation analysis. D-II probably is composed of a unit structure of 4 D-glucose residues and is a glucan consisting of .beta.-D-1,3-linked main chain in which 1 for every 3 D-glucose residues is branched at C-6 with .beta.-D-1,6-linkage.