Novoblocin inhibits initiation of RNA potymerase Il-directed transcription of the moose metaDothiondn-I gene independent of its effect oo DNA topoisomerase II
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (20) , 8547-8559
- https://doi.org/10.1093/nar/15.20.8547
Abstract
The requirement for ATP hydrolysis in the initiation of RNA polymerase II (Pol II)-directed transcription and the relationship between ATP and novobiocin action led us to Investigate whether novoblocin could inhibit transcription of the House netallothioneln-I (MT-I) gene. Novobiocin Inhibited the MT-I gene transcription in a fractionated rat hepatoma nuclear extract In a dose-dependent manner by direct interaction with a nuclear factor(s). This interaction prevented formation of stable prelnitlation complexes but did not affect elongation of MT-I mRNA. Preincubatlon of the nuclear extract with ATP prevented the action of novobiocin on MT-I gene transcription. Although novobiocin is known to Inhibit DNA topolsomerase II, VM-26, a specific inhibitor of this enzyme had no effect on the transcription. These results indicate that novobiocin blocks the Pol Il-directed transcription by Inhibiting formation of preinitiation complexes at an ATP-dependent step.Keywords
This publication has 22 references indexed in Scilit:
- Novoblocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formationin vitroNucleic Acids Research, 1987
- Novobiocin interferes with the binding of transcription factors TFIIIA and TFIIIC to the promoters of class III genesNucleic Acids Research, 1987
- Novobiocin inhibits RNA polymerase III transcriptionin vitroby a mechanism distinct from DNA topoisomerase IINucleic Acids Research, 1986
- Accurate initiation of rat ribosomal RNA gene transcription using a fractionated nuclear extract from normal liver and a hepatoma.Proceedings of the National Academy of Sciences, 1985
- Mechanism of action of DRBJournal of Molecular Biology, 1983
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research, 1983
- Mechanism of RNA polymerase II-specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcriptsCell, 1982
- Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription.Proceedings of the National Academy of Sciences, 1982
- ATP-dependent DNA topoisomerase from D. melanogaster reversibly catenates duplex DNA ringsCell, 1980
- RNA polymerases from a rat hepatoma: Partial purification and comparison of properties with corresponding liver enzymesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976