Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription.
- 1 May 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (10) , 3167-3170
- https://doi.org/10.1073/pnas.79.10.3167
Abstract
The adenosine analog 5,6-dichloro-1-.beta.-D-ribofuranosylbenzimidazole (DRB) and its mono- and triphosphate derivatives inhibit RNA polymerase II-specific transcription in an extract of whole HeLa [human cervical carcinoma] cells. The analog does not inhibit RNA polymerase III-specific adenovirus VA RNA transcription in the whole cell extract. With purified RNA polymerase II under nonspecific transcription conditions, no effect of DRB could be detected. DRB is equally effective in inhibiting in vitro transcription from several of the adenovirus promoters and the human .epsilon.-globin gene. The inhibitory effects are in the order DRB > DRB monophosphate > DRB triphosphate. Thus, DRB acts in vitro presumably on systems in which specific RNA polymerase II initiation of transcription occurs and with no detectable effect on premature termination. This will provide a suitable model for study of the molecular mechanism of action of DRB on transcription.This publication has 25 references indexed in Scilit:
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