Evaluation of the refractometric method for the determination of total protein in avian plasma or serum
Open Access
- 1 July 1985
- journal article
- research article
- Published by Taylor & Francis in Avian Pathology
- Vol. 14 (3) , 441-444
- https://doi.org/10.1080/03079458508436245
Abstract
Serum total protein concentrations in pigeon blood determined with the biuret method (TPB‐se) were compared with total protein concentrations in plasma (TPR‐pl) and serum (TPR‐se) obtained by estimation from refractive index. The refractometric method consistently yielded higher values (P<0.01). A significant correlation between TPB‐se and TPR‐se was found (r = 0.62) but not between TPß‐se and TPR‐pl. The calculated regression equation TPR‐se = 0.69 TPB‐se + 16 was considered to have no practical value, because the correlation coefficient was low (r = 0.62). The refractometric method for determination of TP in pigeon blood is not recommended.Keywords
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