BIOCHEMICAL CHANGES OCCURRING IN A MINIMAL MEDIUM DURING GROWTH AND SPORULATION: COMPARISON OF SPOROGENIC BACILLUS CEREUS WITH ASPOROGENIC MUTANTS

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Abstract
Chemical changes in a minimal synthetic medium were studied during growth and sporulation of normal and temperature-sensitive mutant cultures of B. cereus at 28 °C. Similar chemical studies were made when the cultures were grown at 37 °C; the mutants were asporogenic (nonsporulating) at this temperature.Concurrent events, essentially the relationships between spore formation, the consumption of zinc, and consumption of manganese, were noted in sporulating and nonsporulating cultures. Other components of the medium also investigated included: glucose, amino-N, inorganic-P, potassium, iron, and magnesium. Metabolic products detected in the medium included: diaminopimelic acid, dipicolinic acid, ammonia-N, amide-N, peptide-N, and organic-P.