EFFECT OF AEROSOL IMMUNIZATION WITH RE 595 SALMONELLA MINNESOTA ON LUNG BACTERICIDAL ACTIVITY AGAINST SERRATIA-MARCESCENS, ENTEROBACTER CLOACAE, AND PSEUDOMONAS-AERUGINOSA

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Abstract
Intrapulmonary bactericidal activity was measured after mice were given 3 weekly aerosol exposures to acid-hydrolyzed Re 595 S. minnesota. Ten days after their last immunization, mice were challenged with aerosolized S. marcescens, E. cloacae or P. aeruginosa. Quantitative bacterial counts in ground lung were obtained immediately after exposure and again 4 h later. Enhanced bactericidal activity against S. marcescens and E. cloacae were seen in immunized animals, whereas no difference with P. aeruginosa was noted. In separate studies, immunization with S. marcescens yielded a similar enhancement of lung bactericidal activity. Mucociliary transport, as measured by disappearance of aerosolized S. marcescens labeled with 32P, was identical for immunized and control animals. Using a standardized in vitro mouse alveolar macrophage phagocytic system, lung washes from animals immunized with Re 595 S. minnesota had significant opsonic activity for S. marcescens but not for P. aeruginosa.