NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
Open Access
- 1 December 1965
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 27 (3) , 451-457
- https://doi.org/10.1083/jcb.27.3.451
Abstract
Young wheat roots were labeled with 32P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.This publication has 14 references indexed in Scilit:
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