EFFECT OF 2-ACETYLAMINOFLUORENE ON THE BINDING OF EPIDERMAL GROWTH-FACTOR TO MICROSOMAL AND GOLGI FRACTIONS OF RAT-LIVER CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (7) , 2754-2757
Abstract
The livers of rats fed the hepatocarcinogen 2-acetylaminofluorene [2-AAF] (0.02%) with chow showed a sharp decrease in the binding of epidermal growth factor to microsomes and Golgi fractions. The binding to the latter decreased from 15.3% specific binding per 0.1 mg protein in controls to 9.4% after 2 days and reached a nadir of 0.8% after 21 days. The binding to microsomes decreased from 26.3% specific binding per 0.5 mg protein in the controls to 17.4% after 4 days and reached a nadir of 7.5% after 46 days. The low binding which persisted until the end of the experiment (85 days) was due to the apparent decrease in the number of receptors without significant changes in their affinity. Also, there was only partial recovery in rats fed 2-AAF for 90-107 days and taken off the carcinogen for 30-75 days. In vitro, neither 2-AAF nor its metabolites hydroxy- and acetoxy-2-acetylaminofluorene significantly decreased epidermal growth factor binding to the isolated microsomal fraction.