Measurement of the malondialdehyde–2′‐deoxyguanosine adduct in human urine by immuno‐extraction and liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

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Abstract
The major adduct of malondialdehyde with guanine, M1G, was measured in human urine from non-smoking healthy individuals. M1G is a mutagenic DNA lesion and a terminal product of lipid peroxidation in vivo that may be implicated in cancer related to lifestyle and diet. On the basis of a recently developed method for the quantification of M1G as an excreted deoxynucleoside using immuno-extraction purification, chemical NaBH4 reduction and liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry, we demonstrate that the average 24 h excretion rate of M1G-dR is about 12 ± 3.8 fmol kg−1 (n = 5). Copyright © 2004 John Wiley & Sons, Ltd.

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