Two isozymes of PKC found in HL‐60 cells show a difference in activation by the phorbol ester TPA
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2) , 264-266
- https://doi.org/10.1016/0014-5793(89)80637-4
Abstract
Cytosol from untreated cells and a detergent extract of the particulate fraction from TPA-treated HL-60 cells were analyzed for protein kinase C activity by consecutive column chromatography on Mono Q and hydroxyapatite. From both preparations two separate peaks of enzyme activity were obtained. The first peak, eluting at lower salt concentrations, is activated at lower TPA concentrations (3 × 10−9 M) than the other (10−7 M), which was eluted at higher salt concentrationsKeywords
This publication has 19 references indexed in Scilit:
- Unique substrate specificity and regulatory properties of PKC‐ε: a rationale for diversityFEBS Letters, 1989
- Selective activation of the γ‐subspecies of protein kinase C from bovine cerebellum by arachidonic acid and its lipoxygenase metabolitesFEBS Letters, 1989
- Effects of activators of protein kinase C, including bryostatins 1 and 2, on the growth of A549 human lung carcinoma cellsInternational Journal of Cancer, 1989
- Identification of three additional members of rat protein kinase C family: gd‐, ϵ‐ and ξ‐subspeciesFEBS Letters, 1987
- Immunochemical identification of protein kinase C isozymes as products of discrete genesBiochemical and Biophysical Research Communications, 1987
- Comparative effects of polymyxin B, phorbol ester and bryostatin on protein phosphorylation, protein kinase C translocation, phospholipid metabolism and differentiation of HL60 cellsBiochemical and Biophysical Research Communications, 1987
- Inositol lipids and cell proliferationBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1987
- Bryostatin 1, an activator of protein kinase C, inhibits tumor promotion by phorbol esters in SENCAR mouse skinCarcinogenesis: Integrative Cancer Research, 1987
- The Complete Primary Structure of Protein Kinase C—the Major Phorbol Ester ReceptorScience, 1986
- Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membraneNature, 1983