DETERMINATION OF AMPROLIUM, ETHOPABATE, LASALOCID, MONENSIN, NARASIN, AND SALINOMYCIN IN CHICKEN TISSUES, PLASMA, AND EGG USING LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

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Abstract
A liquid chromatographic-atmospheric pressure ionization ion spray method for the determination of some coccidiostatics in chicken egg, fat, liver, muscle, and plasma is presented. The samples were extracted with acetone-tetrahydrofuran. The water and the organic layer was then separated using a liquid-liquid extraction step and the organic phase was then evaporated to dryness. The dry residue was diluted in hexane and a portion of the organic phase was cleaned-up with a simple step for ethopabate analyses while the rest of the organic phase was purified using a silica solid phase extraction column to analyse lasalocid, monensin, narasin, and salinomycin. The water phase (amprolium analyses) was diluted with water, filtered, and injected into the LC-MS. Three different analytical columns were used. The detection limits were from 1 ng/g to 7 ng/g for chicken tissue and from 4 to 10 ng/mL for plasma.

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