Use of Urea and Salicylate to Elute Antibody from Insoluble Antigen.

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Abstract
Solutions of 16-32% urea and of 10% sodium salicylate were used to separate I131 labeled rabbit anti-rat organ antibodies from insoluble rat organ residues acting as antigens. Following removal of these eluting agents, the antibody retained its specificity for rat organs as measured by in vivo and in vitro technics. Measured by in vivo technics, rabbit gamma globulin was resistant to the altering or denaturing effect of these reagents.
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