ENZYMATIC BASIS OF RESISTANCE TO AUREOMYCIN III. Escherichia coli

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Abstract
Bovine serum albumin and certain other proteins stimulate up to 2000% the rate of reduction of 2,6-dichlorophenolindophenol mediated by highly purified extracts of Aureomycin (chlortetracycline)-sensitive and -resistant Escherichia coli. Unstimulated reductions in extracts derived from sensitive cells are sensitive to Aureomycin, whereas those catalyzed by extracts of resistant cells are resistant to the antibiotic. No such parallelism exists in the stimulated reductions. In this instance, both sensitive and resistant extracts are sensitive to Aureomycin. The stimulation is pH-dependent and is inhibited only by certain metal binders. Possible mechanisms of the stimulatory effect and the relationship of the phenomenon both to sensitivity of bacterial cells to Aureomycin and the development of resistance thereto are discussed.