The intracellular cytoplasmic domain of the Alzheimer's disease amyloid precursor protein interacts with phosphotyrosine‐binding domain proteins in the yeast two‐hybrid system
- 18 November 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 397 (2-3) , 197-200
- https://doi.org/10.1016/s0014-5793(96)01128-3
Abstract
We have used the yeast two-hybrid system to screen for proteins that interact with the carboxy-terminal domain of APP. Six different clones were isolated and sequence analyses revealed that they encoded domains of a previously described neuronal protein Fe65, a homologue of Fe65 and a homologue of protein X11. All of these proteins contain one or more phosphotyrosine binding (PTB) domains. PTB domain proteins bind to the sequence Asn-Pro-X-Tyr when the Tyr is phosphorylated and are believed to function in signal transduction. APP contains such a motif. These results are consistent with a role for APP in signal transduction mechanisms.Keywords
This publication has 27 references indexed in Scilit:
- 764 A new signal transduction pathway in Alzheimer's diseaseNeurobiology of Aging, 1996
- The Regions of the Fe65 Protein Homologous to the Phosphotyrosine Interaction/Phosphotyrosine Binding Domain of Shc Bind the Intracellular Domain of the Alzheimer's Amyloid Precursor ProteinJournal of Biological Chemistry, 1995
- The Amyloid Peptide and Its Precursor in Alzheimer's DiseaseReviews in the Neurosciences, 1995
- An Alternative to SH2 Domains for Binding Tyrosine-Phosphorylated ProteinsScience, 1994
- Normal and Abnormal Biology of the beta-Amyloid Precursor ProteinAnnual Review of Neuroscience, 1994
- Role of the β-amyloid precursor protein in Alzheimer's diseaseTrends in Biochemical Sciences, 1994
- Alzheimer amyloid protein precursor complexes with brain GTP-binding protein GoNature, 1993
- An Endosomal‐Lysosomal Pathway for Degradation of Amyloid Precursor Protein aAnnals of the New York Academy of Sciences, 1992
- GAL4 fusion vectors for expression in yeast or mammalian cellsGene, 1992
- Processing of the Amyloid Protein Precursor to Potentially Amyloidogenic DerivativesScience, 1992