ISOLATION AND CHARACTERIZATION OF FC-RECEPTORS SHED FROM HUMAN PERIPHERAL MONONUCLEAR-CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 35  (3) , 559-566
Abstract
Supernatants of human peripheral mononuclear cells containing membrane components shed as a consequence of 4-37.degree. temperature shifts were used as source for isolation of Fc-receptors (FcR). Aggregated Ig[immunoglobulin]G1 myeloma protein and TMV[tobacco mosaic virus]-anti-TMV complexes proved suitable sorbents to adsorb quantitatively and specifically the FcR. The isolated FcR interacted only with IgG and not with IgM. No hemagglutination was observed when the isolated FcR was incubated with sensitized human Rh+ red blood cells. Complement dependent lysis of sheep red blood cells was not inhibited by the isolated FcR. The interaction between IgG and SpA from Staphylococcus aureus (Cowan I) bacteria was not inhibited when red blood cells sensitized with IgG were preincubated with the isolated FcR. The differences between the FcR-like material isolated from supernatants of peripheral human mononuclear cells and those secreted by stimulated T [thymus-derived] cells or produced by lymphoblastoid cells lines are discussed.