OXIDATION OF 2-DEOXY- d -GLUCOSE TO 2-DEOXY- d -GLUCONIC ACID BY EXTRACTS OF PSEUDOMONAS AERUGINOSA

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Abstract
A cell-free extract of P. aeruginosa was shown to contain constitutive enzyme systems capable of oxidizing 2-deoxy-D-glucose to 2-deoxy-D-gluconic acid. This reaction was analogous to the oxidation of glucose to gluconic acid by this microorganism since no phosphorylated intermediates could be demonstrated and di- or tri-phosphopyridine nucleotide was not involved. Two enzyme systems were found in sonic extracts which were capable of oxidizing 2-deoxy-D-glucose. One fraction, precipitated between 0 and 35% ammonium sulfate saturation, was active on both glucose and 2-deoxy-D-glucose. The activity for 2-deoxy-D-glucose in this fraction could not be separated from glucose dehydrogenase. Another fraction obtained from the system at 40 to 60% ammonium sulfate saturation was active on 2-deoxy-D-glucose but inactive on glucose.