Combined Bright Field and Fluorescence Staining of Paraffin Sections for Studying the Fertilization Process in Plants
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 56 (3) , 195-198
- https://doi.org/10.3109/10520298109067310
Abstract
PAS-toluidine blue O—aniline blue staining of paraffin sections allows study of histological and cytological detail while retaining aniline blue induced fluorescence in all “callose sites”. Because most autofluorescence is eliminated by the PAS-toluidine blue prestaining, the detail and contrast of the fluorescence image is superior to slides stained in aniline blue alone. Slides are stained by the PAS reaction, 0.03% toluidine blue O, alkaline 0.005% aniline blue, and mounted directly in aqueous mounting medium.This publication has 13 references indexed in Scilit:
- Cryptic Self-Fertilization in the MalpighiaceaeScience, 1980
- A critical evaluation of the specificity of aniline blue induced fluorescenceProtoplasma, 1978
- Reduced pollen tube growth and the presence of callose in the pistil of the male floral stage of the avocadoScientia Horticulturae, 1977
- CONTROL BY THE EMBRYOSAC OVER POLLEN TUBE GROWTH IN THE STYLE OF THE AVOCADO (PERSEA AMERICANA MILL.)New Phytologist, 1976
- A Fluorescent Brightener used for Pollen Tube IdentificationIn VivoStain Technology, 1974
- Sequential Staining of Callose by Aniline Blue and Lacmoid for Fluorescence and Regular Microscopy on a Durable Preparation of the Same SpecimenStain Technology, 1973
- Euparal as A Mounting Medium for Preserving Fluorescence of Aniline Blue in Plant MaterialStain Technology, 1973
- Observing pollen tubes by means of fluorescenceEuphytica, 1968
- Plant Microtechnique: Some Principles and New MethodsAmerican Journal of Botany, 1968
- Staining and Observing Pollen Tubes in the Style by Means of FluorescenceStain Technology, 1959