The Effect of Sodium Copper Chlorophyllin on Pure Cultures of Oral-Type Organisms

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Abstract
The application of standard testing methods has demonstrated a bacteriostatic action by chlorophyll on pure cultures of staphlococci, streptococci and lactobacilli. Concurrent studies revealed that on a unit cell basis, glycolysis was not adversely affected and may be stimulated. The irreversibility of the chlorophyllin inhibition by required vitamins and salts in 10 and 100 times the minimal concentrations required for growth was established with Streptococcus salivarius (American Type Culture Collection No. 9756) in Smiley''s synthetic medium. Experiments conducted using a Warburg respirometer showed chlorophyllin interference with the normal utilization of oxygen by Str. salivarius and 5 strains of staphylococci. The addition of chlorophyllin in various concentrations to Smiley''s synthetic medium showed that the oxidation-reduction potential of the medium could be altered as much as 100 millivolts in favor of oxidation when tested at pH 7.0 and 30[degree]C, using a Beckman potentiometer with a Pt electrode.

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