EFFECT OF INTERFERON ON PLURIPOTENT HEMATOPOIETIC PROGENITORS (CFU-GEMM) DERIVED FROM HUMAN-BONE MARROW

  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (7) , 587-590
Abstract
A culture assay for multilineage hemopoietic progenitors (CFU-GEMM) that form mixed hematopoietic colonies containing granulocytes, erythroblasts, megakaryocytes and macrophages was described. The effect of human leukocyte interferon on multilineage hemopoietic progenitors was examined. [Interferons have been proposed as antineoplastic agents and are being used in clinical trials for patients with acute and chronic leukemia, lymphoma and numerous other tumors.] Leukocyte interferon did reduce the plating efficiency of noncommitted precursors (CFU-GEMM) in a dose-related fashion. A reduction of the plating efficiency of mixed hemopoietic colonies was observed even when interferon was added 72 h after initiation of the cultures.