Abstract
Enzyme-linked immunosorbent assay (ELISA) was tested for the ability to differentiate between botulinum type C1 and D toxins. The results indicate an antigenic relationship between type C1 and D toxins. Indications were obtained that the antigenicities of type C1 and of D toxins produced by different strains are not identical. Although ELISA can be used to differentiate type C1 and D toxins from those of other types, the assay has only limited value to differentiate between type C1 and D toxins. No parallel relationship between toxicity (mouse bioassay) and toxin-related-antigen contents (ELISA) of Clostridium botulinum type C and D cultures was found.