EARLY CHANGES IN PHOSPHATIDYLCHOLINE METABOLISM IN HUMAN ACUTE PROMYELOCYTIC LEUKEMIA-CELLS STIMULATED TO DIFFERENTIATE BY PHORBOL ESTER
- 1 January 1981
- journal article
- research article
- Vol. 58 (2) , 237-243
Abstract
The HL-60 leukemia cell line derived from a human acute promyelocytic leukemia is stimulated to differentiate into macrophages within 24-48 h after exposure to the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) [a carcinogen]. Early alterations (within 90 min of exposure to TPA) in phosphatidylcholine metabolism were studied in HL-60 cells. Phosphatidylcholine synthesis by methylation of phosphatidylethanolamine was inhibited in a dose-dependent fashion. Synthesis of phosphatidylcholine from endogenous choline was enhanced and correlated inversely with the degree of inhibition of the methylation pathway. Phorbol ester congeners of TPA caused similar alterations in phosphatidylcholine metabolism in direct relationship to their capacity to induce differentiation in HL-60 cells. Perturbation of phosphatidylcholine metabolism is an early membrane event in TPA-induced HL-60 cell differentiation.This publication has 31 references indexed in Scilit:
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