Hormone-sensitive lipase in brown adipose tissue: Identification and effect of cold exposure
- 1 November 1987
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 7 (11) , 897-904
- https://doi.org/10.1007/bf01119481
Abstract
Hormone-sensitive lipase (HSL) in brown adipose tissue from mice was identified through immunoprecipitation with a polyclonal antibody (anti-HSL) towards rat white fat HSL and Western blotting. An 82 kDa polypeptide, slightly smaller than the rat white fat HSL 84 kDa subunit, was detected and its identity as HSL verified by inhibition properties. The HSL concentration per g tissue was several-fold higher in the mouse brown adipose tissue than in the rat white adipose tissue, but the specific activities per mg protein were similar. Cold-exposure (4°C of the mice for 24 h approximately doubled the HSL concentration but this increase parallelled the overall protein increase and did not reflect a specific effect on the HSL.This publication has 25 references indexed in Scilit:
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