Stereochemistry of catabolism of the RNA base uracil
- 1 January 1985
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 7,p. 1355-1362
- https://doi.org/10.1039/p19850001355
Abstract
A mammalian enzyme system has been used to study the stereochemistry of the catabolism of the pyrimidine uracil (1) to the amino acid β-alanine (4). Use of [5-2H]- and [6-2H]-uracils and of 2H2O in the incubations yielded stereospecifically deuteriated samples of β-alanine. Assays, involving total synthesis of samples of β-alanine unambiguously labelled with deuterium in each of the four C–H bonds have shown that, in the first step in the catabolic process, uracil is reduced by dihydrouracil dehydrogenase with overall anti addition of hydrogen, at the si face at C-6 and the si face at C-5.This publication has 4 references indexed in Scilit:
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