The biosynthesis and metabolism of oestrogens in the rat. 1. Conversion of [4-14C]testosterone into [14C]oestradiol-17β by the rat ovary

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Abstract
The biosynthesis of estradiol-17[beta] from [4-C14]testosterone by rat ovarian tissue has been demonstrated in vitro. The specific activity of the estradiol isolated was followed through multiple purifications by chromatography on columns and on paper, precipitation with digitonin, 6 recrystallizations in 2 different solvent systems followed by partition chromatography, and by methylation, purification of the methyl ethers and chromatography in 2 successive systems. The biosynthesis of at least 5 radioactive compounds in the phenolic fraction was indicated. The lack of evidence for this biosynthesis as a significant biochemical process in vivo is emphasized.