A Circadian Enhancer Mediates PER-Dependent mRNA Cycling in Drosophila melanogaster
Open Access
- 1 July 1997
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 17 (7) , 3687-3693
- https://doi.org/10.1128/mcb.17.7.3687
Abstract
Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.Keywords
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