7‐Deaza purine bases offer a higher ion stability in the analysis of dna by matrix‐assisted laser desorption/ionization mass spectrometry
- 1 January 1995
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 9 (6) , 525-531
- https://doi.org/10.1002/rcm.1290090611
Abstract
Oligodeoxynucleotides which contain 7‐deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified Oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI‐MS with positive ion detection mode are presented. Additionally, the potential use of 7‐deaza purine nucleotides in the MALDI‐MS analysis of DNA sequencing reactions is discussed.Keywords
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