Specific cleavage analysis of mammalian mitochondrial DNA.
Open Access
- 1 November 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (11) , 4496-4500
- https://doi.org/10.1073/pnas.72.11.4496
Abstract
Mitochondrial DNA from several mammalian species has been digested with a site-specific restriction endonuclease (HaeIII) from Haemophilus aegyptius. A quantitative analysis of the resulting specific fragments indicates that the mtDNA of any individual mammal is predominantly a single molecular clone. Gel analysis of specific cleavage products has proven quite sensitive in detecting differences in mtDNA: mtDNAs from the more distantly related mammals studied (e.g., donkey and dog) are found to have few bands in common and very closely related mammals (e.g., donkey and horse) share only about 50% of their bands. This procedure has detected several intraspecies mtDNA differences. Six distinct human patterns have been found, with one pattern usually differing from another in two or three bands. mtDNAs from different organs of single individuals have also been analyzed, and no differences have been found.Keywords
This publication has 17 references indexed in Scilit:
- Restriction Endonuclease Cleavage Maps of Animal Mitochondrial DNAsProceedings of the National Academy of Sciences, 1974
- Hemophilus aegyptius restriction endonuclease cleavage map of the simian virus 40 genome and its colinear relation with the Hemophilus influenzae cleavage map of SV40Journal of Molecular Biology, 1974
- A map of simian virus 40 transcription sites expressed in productively infected cellsJournal of Molecular Biology, 1973
- Isolation and Genetic Localization of Three ϕX174 Promoter RegionsNature New Biology, 1973
- Mapping of new Escherichia coli K and 15 restriction sites on specific fragments of bacteriophage phi X174.1973
- Specific Origin in SV40 DNA ReplicationNature New Biology, 1972
- Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzaeProceedings of the National Academy of Sciences, 1971
- A Novel Closed-Circular Mitochondrial DNA with Properties of a Replicating IntermediateProceedings of the National Academy of Sciences, 1971
- The circularity of mitochondrial DNA.Proceedings of the National Academy of Sciences, 1966
- CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA)The Lancet, 1964