Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test

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Abstract
Serological properties of antigens extracted from strains of H. pleuropneumoniae belonging to 7 serotypes were investigated. Antisera were prepared in rabbits against formalin-treated whole cell suspensions and autoclaved cell suspensions. Saline and heat extracts and their alcohol precipitate antigens of H. pleuropneumonia were used in the indirect hemagglutination test. All the antigens used were easily adsorbed directly onto sheep erythrocytes. Saline extract antigen showed maximum type specificity. Heating of the whole cell suspension revealed the cross-reactive minor antigenic determinants. Thus, the heat extract preparations had type-specific and species-specific antigens. Apparently, the indirect hemagglutination test is useful for serotyping and serodiagnosis of H. pleuropneumoniae infections in pigs.