A New Technique for Maintaining and Monitoring Conscious, Stress-free Rabbits in a Steady State: Its Use in the Determination of Glucose Kinetics

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Abstract
A new technique is described for maintaining and monitoring conscious rabbits in a steady state. Solutions were infused and blood samples were withdrawn without major surgery and without causing stress. Cannulation, for infusion and blood sampling, was via the ear veins and arteries. The validity of the technique is discussed with special reference to the amount of blood removed from the animal, use of a plasma substitute solution, use of heparin and assessing whether the animal was in a steady state with respect to glucose. The technique was used to determine glucose kinetics in the fasted rabbit after an injection of [2-3H]glucose with subsequent blood sampling over 2 h. A 2 compartment model was used to calculate certain physiological values, the 2 principal ones being the glucose uptake rate (3.30 .+-. 0.24 (13) mg .cntdot. kg-1 .cntdot. min-1) and the initial volume of distribution (74.8 .+-. 7.8 (13) ml .cntdot. kg-1). The accuracy and precision of the results are discussed.