Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections

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Abstract
High resolution growth recording techniques and reverse isotope dilution analysis were used to study the relationship between IAA concentration and curvature of excised bean (P. vulgaris L. cv Bush Burpee Stringless) 1st internode sections unilaterally treated with hormone. The maximum rate of curvature occurred rapidly (within 25 min) and was proportional to the log of the amount of applied IAA recovered in the tissue. The rate of curvature decreased after 30 min although little or no lateral migration of applied IAA occurred and tissue levels of IAA increased. The biologic activity of IAA-amino acid conjugates was directly related to the amount of free IAA, resulting from their hydrolysis, which could be recovered from the tissue.