Characterization of Araujia Mosaic Virus by In Vitro Translation Analyses

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Abstract
Araujia mosaic virus (AjMV), previously described as a possible new potyvirus group member, was partially purified and compared further with other potyviruses by in vitro translation analyses. Isolated AjMV RNA was translated in a rabbit reticulocyte lysate system, and the translation products were tested for their relatedness to other potyviral proteins by immunoprecipitation tests followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two AjMV RNA translation products, with estimated MW of 49,000 daltons (49kd) and 53kd, were similar in size and serological reaction to tobacco etch virus (TEV) nuclear inclusion proteins. Antiserum to TEV capsid protein reacted with the presumed AjMV capsid protein while antiserum to TEV cylindrical inclusion protein did not react with any of the AjMV translation products. Antiserum to dasheen mosaic virus cylindrical inclusion protein reacted with the presumed AjMV cylindrical inclusion protein produced in vitro. Antiserum to tobacco vein mottling virus helper component protein reacted with an 81kd AjMV translation product. A proposed gene order of translation for AjMV genome is as follows: 5'' end 81kd helper component-related protein-49Kd protein-40Kd protein-70Kd cylindrical inclusion protein -53kd protein-32kd capside protein-3'' end. Evidently, AjMV is a distinct member of the potyvirus group.