A Simplified Technic of Spectrography and Its Application to the Study of Intracellular Hemoglobin

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Abstract
1. A simplified microspectrograph for use in the visible range is described. 2. A nonrecording microdensitometer for use with the above is discussed. 3. The Soret band of hemoglobin is demonstrable in individual erythrocytes and is not affected by fixing or staining. 4. The ratio of the hemoglobin concentration to cosin staining is constant in erythrocytes and the cytoplasm of immature red cells but not in the nucleus. 5. There is no detectable hemoglobin or verdoperoxidase in the granules present in the eosinophile granulocytes of the camel. 6. Both dried and fixed and stained erythrocytes can take up carbon monoxide from the atmosphere.