Low frequency MFs increased inositol 1,4,5‐trisphosphate levels in the Jurkat cell line
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3) , 151-154
- https://doi.org/10.1016/0014-5793(95)00031-4
Abstract
We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T-cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol., 156 (1993) 395–398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5-trisphosphate (IP3) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP3 induced by MF. This result implied that MF-induced Ca2+ oscillations were not due to direct stimulation of the Ca2+-dependent phospholipase C-γ1 (PLC-γ1)Keywords
This publication has 30 references indexed in Scilit:
- Involvement of the brain type of ryanodine receptor in T‐cell proliferationFEBS Letters, 1994
- Experimental evidence for 60 Hz magnetic fields operating through the signal transduction cascadeFEBS Letters, 1993
- Signal transduction events leading to T-cell lymphokine gene expressionImmunology Today, 1993
- Pulsed magnetic field effects on calcium signaling in lymphocytes: Dependence on cell status and field intensityFEBS Letters, 1992
- Delineation of electric and magnetic field effects of extremely low frequency electromagnetic radiation on transcriptionBiochemical and Biophysical Research Communications, 1991
- Nonthermal 60 Hz sinusoidal magnetic‐field exposure enhances 45Ca2+ uptake in rat thymocytes: dependence on mitogen activationFEBS Letters, 1990
- Effects of 50-Hertz electromagnetic fields on proliferation and on chromosomal alterations in human peripheral lymphocytes untreated or pretreated with chemical mutagensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1989
- Regulation of receptor-mediated calcium influx across the plasma membrane in a human leukemic T-cell line: evidence of its dependence on an initial calcium mobilization from intracellular storesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1988
- Effect of low‐level, 60‐Hz electromagnetic fields on human lymphoid cells: I. Mitotic rate and chromosome breakage in human peripheral lymphocytesBioelectromagnetics, 1986
- A role for Ca2+ in the effect of very low frequency electromagnetic field on the blastogenesis of human lymphocytesFEBS Letters, 1985