Oxygen-Linked Hydrogen Ion Binding of Human Hemoglobin. Effects of Carbon Dioxide and 2,3-Diphosphoglycerate
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 29 (2) , 185-193
- https://doi.org/10.3109/00365517209081074
Abstract
The Bohr coefficient, b, and the Haldane coefficient, h, were measured simultaneously in erythrolysates in the presence and absence of CO2and 2,3-diphosphoglycerate. We found b—h=—0.04 (—0.023 to —0.054) although b—h=—0 according to the linkage equation. The cause of the observed difference seems to be that his measured as the mean value for oxygen saturation fractions (zHbO2) between 0 and 1, while brefers to a zHbO2of 0.54. Apparently b(and h) varies with zHbO2and reaches a numerical maximum at an intermediate zHbO2value.Keywords
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