UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis
- 1 March 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (5) , 1810-1812
- https://doi.org/10.1128/jb.173.5.1810-1812.1991
Abstract
Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.This publication has 18 references indexed in Scilit:
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