Studies on polypeptides XVIII Solid‐phase synthesis of RNase S‐peptide analogues with high S‐protein activating ability; replacement of histidine‐12 by L‐homohistidine
- 1 January 1976
- journal article
- research article
- Published by Wiley in Recueil des Travaux Chimiques des Pays-Bas
- Vol. 95 (11) , 278-281
- https://doi.org/10.1002/recl.19760951108
Abstract
As part of a structure‐activity relationship study of ribonuclease, focussing on the role of histidine‐12 in the active centre, the following polypeptides have been synthesized by the solid‐phase method and tested for S‐protein activating ability: bovine RNase S‐peptide 1−14, [Ile13]‐ S‐peptide 1−14 and the corresponding analogues in which histidine‐12 is replaced by L‐homohistidine. Whereas their binding tendency is considerably lower the [Hhi12] analogues show a remarkably high ability to generate enzymatic activity when combined with S‐protein in the presence of yeast RNA as a substrate.Funding Information
- Netherlands Foundation of Chemical Research
- Netherlands Organization for the Advancement of Pure Research
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