STUDIES ON GRAFT VERSUS HOST (GVH) REACTIONS .1. IMPAIRMENT OF HEMATOPOIETIC STROMA IN MICE SUFFERING FROM GVH DISEASE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 9  (2) , 101-110
Abstract
Hematologic changes were described in adult (C57BL/6 .times. C3H/He) F1 hybrid mice suffering from graft vs. host (GvH) disease induced with 108 parental (C3H/He) lymph node (PLN) cells. Progressive decrease in numbers of leukocytes, granulocytes, erythrocytes and reticulocytes was observed from day 7 to 14 of GvH disease. Thrombocytopenia was not so remarkable. A histologic study revealed an extremely hypoplastic marrow. The number of colony-forming units (CFUS[pluripotential stem cells])/femur decreased progressively from day 5-14. When the femurs, removed from the diseased mice at various intervals after the PLN cell injection, were implanted s.c. into other hosts for 7 wk to evaluate the hemopoietic stroma to support the hemopoietic stem cells, the progressive decrease in CFUS number in the implanted femurs was also observed from day 5-14. There was a dose relation between the number of PLN cells injected and the reduction of CFUS number in the femurs in situ or the implanted femurs. The impairment of hemopoietic stroma apparently results from F1 hybrid disease, i.e., GvH disease, and can be one of the major causes of the depletion of hemopoietic cells resulting in pancytopenia.