Identification and radioimmunologic estimation of sexual steroid hormones in aqueous humor and vitreous of rabbit eye
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Albrecht von Graefes Archiv für Ophthalmologie
- Vol. 199 (3) , 261-266
- https://doi.org/10.1007/bf00417296
Abstract
The occurrence of testosterone, estrone, and estradiol-17α in aqueous humor and vitreous body of the rabbit eye was demonstrated. The identity of the steroid hormones was confirmed by their chromatographic properties, microreactions, and radiochemically by crystallization to constant specific activity of their labeled acetates. The levels of testosterone and estrogens in rabbit male and female plasma, aqueous, and vitreous were determined by radioimmunologic assay. The concentrations of the steroid hormones in plasma are higher than in aqueous and vitreous. However, testosterone in aqueous circulates in free form and lacks the binding to sexual hormone-binding globulin as in plasma. Bericht über das Vorkommen von Testosteron, Oestron und Oestradiol-17α im Kammerwasser und Glasörper des Kaninchenauges. Die Art der Steroide wurde mittels Chromatographie, Mikroreaktionen und radiochemisch ermittelt. Der Testosteron- und Oestrogenspiegel im männlichen und weiblichen Kaninchenplasma, im Kammerwasser und Glaskörper wurde radioimmunologisch bestimmt. Die Plasmakonzentration der Steroidhormone lag höher als diejenige im Kammerwasser oder Glaskörper. Testosteron zirkuliert jedoch im Kammerwasser in freier Form, im Plasma dagegen ist es überwiegend an Sexualhormonebindende Globuline gebunden.This publication has 15 references indexed in Scilit:
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