COMPARATIVE STUDIES ON METHODS OF ASSAY FOR A ROUTINE, CLINICAL GONADOTROPHIN DETERMINATION IN HUMAN URINE

Abstract
The alcohol precipitation and the kaolin adsorption extraction methods for the estimation of urinary gonadotrophin were compared using samples from 99 human patients. Both extraction procedures gave satisfactory results, but for routine clinical tests, a rapid and inexpensive modification of the kaolin adsorption method is suggested. The rat ovary test, in contradistinction to the mouse uterus test, was not found suitable for low gonadotrophin levels as it is not sufficiently sensitive. The results are discussed and a routine clinical method for the determination of gonadotrophin in human non-pregnant urine is proposed.