An Antibody to theDrosophila PeriodProtein Labels Antigens in the Suprachiasmatic Nucleus of the Rat
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Neurogenetics
- Vol. 8 (1) , 33-42
- https://doi.org/10.3109/01677069209167270
Abstract
Cell bodies in the rat suprachiasmatic nucleus (SCN) were labeled with an antibody against a small domain of the period (per) protein, the product of a gene in Drosophila that regulates circadian rhythms. In immunoblots of SCN protein extracts, the antibody recognized a band of approximately 115 kD, as well as a heterogeneous antigen ranging from 160 kD to 170 kD. The antibody was found in previous studies to label putative circadian pacemaker neurons in Aplysia and Bulla, as well as the cellular sites of per expression in flies. Taken together, these results suggest that the region of the per protein recognized by this antibody may be widely conserved in neuronal circadian pacemakers.Keywords
This publication has 30 references indexed in Scilit:
- Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levelsNature, 1990
- Genetics of Biological Rhythms in DrosophilaPublished by Elsevier ,1990
- Synaptic inputs and electrical coupling among magnocellular neuroendocrine cellsBrain Research Bulletin, 1988
- Mutations and Molecules Influencing Biological RhythmsAnnual Review of Neuroscience, 1988
- Genetic and Molecular Analysis of Biological RhythmsJournal of Biological Rhythms, 1987
- The Drosophila clock gene per affects intercellular junctional communicationNature, 1987
- A family of unusually spliced biologically active transcripts encoded by a Drosophila clock geneNature, 1987
- Role of the Optic Lobes in the Regulation of the Locomotor Activity Rhythm ofDrosophila melanogaster: Behavioral Analysis of Neural MutantsJournal of Neurogenetics, 1986
- Differential Responses to Osmotic Stress of Vasopressin-Neurophysin mRNA in Hypothalamic NucleiNeuroendocrinology, 1984
- Circadian rhythm of the locomotor activity in Drosophila melanogaster and its mutants ‘sine oculis’ and ‘small optic lobes’Physiological Entomology, 1983