Simultaneous Synthesis of Multiple Oligonucleotides Using Nucleoside-H-Phosphonate Intermediates
- 1 November 1990
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 9 (9) , 691-696
- https://doi.org/10.1089/dna.1990.9.691
Abstract
A semimechanized simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonates as intermediates was developed and applied to the preparation of hybridization probes. The synthesis could be done with equivalent results (about 98% average yield per cycle) using regenerated H-phosphonates.This publication has 17 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Polymer support synthesis : XII. Derivatization with the 4-decyloxytrityl group as an aid in the affinity chromatography of oligo- and polynucleotidesJournal of Chromatography A, 1987
- Studies on the Synthesis of Oligonucleotidesviathe Hydrogenphosphonate ApproachNucleosides and Nucleotides, 1987
- Semi-Mechanized Simultaneous Synthesis of Multiple Oligonucleotide FragmentsNucleosides and Nucleotides, 1987
- A System for the Simultaneous Chemical Synthesis of Different DNA Fragments on Solid SupportDNA, 1986
- Sequence of the cDNA encoding porcine pro-opiomelanocortinBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986
- Nucleoside Phosphotransferase from Malt Sprouts. I. Isolation, Characterization and Specificity of the EnzymeBiological Chemistry Hoppe-Seyler, 1986
- Nucleoside h-phosphonates: Valuable intermediates in the synthesis of deoxyoligonucleotidesTetrahedron Letters, 1986
- Filter disc supported oligonucleotide synthesis by the phosphite methodNucleic Acids Research, 1984
- Studies on Polynucleotides. XXIV.1 The Stepwise Synthesis of Specific Deoxyribopolynucleotides (4).2 Protected Derivatives of Deoxyribonucleosides and New Syntheses of Deoxyribonucleoside-3″ Phosphates3Journal of the American Chemical Society, 1963